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    Structured Review

    ATCC m46753 emem media atcc
    M46753 Emem Media Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m46753+emem+media+atcc/Eagle's+Minimum+Essential+Medium/pm39084225-207-82-85
    Average 99 stars, based on 6331 article reviews
    m46753 emem media atcc - by Bioz Stars, 2026-09
    99/100 stars

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    Recombinant:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..

    SYBR Green Assay:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..

    Staining:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..

    Control:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..

    Cell Culture:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..

    Cryo-EM Sample Prep:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..

    Binding Assay:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..

    Mass Spectrometry:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..

    Purification:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..

    Targeted Proteomics:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..

    Filtration:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..

    CRISPR:

    Article Title: Identification of potent and reversible piperidine carboxamides that are species-selective orally active proteasome inhibitors to treat malaria.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains One Shot TOP10 Chemically Competent E. coli Invitrogen Cat# C404003 Biological samples Male O+ red blood cells (single donor, anonymized) BioIVT Cat# HumanRBCPD-0120194 Fetal Bovine Serum (FBS) R&D Systems/bio-techne Cat# S11150 Human liver microsomes (pooled 100 male and 100 female donors) XenoTech LLC Lot # 1910096 Rat liver microsomes (pooled male, 711 donors) XenoTech LLC Lot # 1910100 Human cryopreserved hepatocytes (pooled male and female, 5 donors each) XenoTech LLC Custom Lot # 2110272 Rat cryopreserved hepatocytes (pooled male, 6 donors) XenoTech LLC Lot # 2110189 Human whole blood (single donor, anonymized) Volunteer Blood Donor Registry, The Walter and Eliza Hall Institute, Parkville, Victoria, Australia N/A Human plasma (pooled, mixed gender, Na Heparin as anticoagulant) Innovative Research, Novi, MI Lot # 31992 Rat plasma (Sprague-Dawley, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1612 Mouse plasma (CD1, pooled, mixed gender, Na Heparin as anticoagulant) Valley Biomedical, Winchester, VA Lot # 20J1613 Chemicals, peptides, and recombinant proteins (rac)-SW042 This paper N/A (S)-SW042, This paper N/A (R)-SW042 This paper N/A SW858 This paper N/A SW923 This paper N/A SW931 This paper N/A SW996 This paper N/A SW726 This paper N/A SW723 This paper N/A SW143 This paper N/A SW584 This paper N/A Bortezomib Sigma-Aldrich Cat # 5043140001 MG-132 Sigma-Aldrich Cat# 474790-20MG Epoxomicin Sigma-Aldrich Cat# 324800 Dihydroartemisinin Sigma-Aldrich Cat# 1200520 Succinyl-Leu-Leu-Val-Tyr-AMC Bio-Techne Cat# S-280 Dextromethorphan Sigma-Aldrich Cat# D9684 Diclofenac Sigma-Aldrich Cat# D6899 Omeprazole Sigma-Aldrich Cat# O104 Phenacetin Fluka Cat# 77440 Verapamil Aldrich-Aldrich Cat# 381195 Midazolam Sigma-Aldrich Cat# M2419 Lucifer yellow Sigma-Aldrich Cat# L0259 Rhodamine 123 Sigma-Aldrich Cat# R8004 (Continued on next page) e1 Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Diazepam Sigma-Aldrich Cat# D0899 Warfarin Sigma-Aldrich Cat# A2250 cbZ-Leu-Leu-Glu-AMC Bio-Techne Cat# S-230 Acetate-Arg-Leu-Arg-AMC Cayman chemicals Cat# 26643 WLW-vs This paper N/A MV151 This paper N/A DSM265 Phillips et al., ref. 13 N/A Human 20S Immunoproteasome South Bay Bio Cat# SBB-PP0004 Human 20S proteasome South Bay Bio Cat# SBB-PP0005 Recombinant human PA28 Activator alpha Subunit protein Bio-Techne Cat# E 381 SYBR Green I nucleic acid gel stain Sigma-Aldrich Cat# S9430 MitoTracker Deep Red FM Dye Thermo Fisher Cat# M46753 EMEM media ATCC Cat# 30-2003 RPMI media Gibco/life/ThermoFisher Cat# 11875135 DMEM media Gibco/life/ThermoFisher Cat#10566016 Brefeldin A LC Labs Cat# B-8500 b-mercaptoethanol Sigma-Aldrich Cat# M3148 Pen/Strep Gibco/life/ThermoFisher Cat# 15140163 L-glutamine Gibco/life/ThermoFisher Cat# 25030081 Critical commercial assays CellTiter Glo Promega Cat# G7573 MycoAlert detection kit Lonza, Walkersville MD Cat#LT07-710 MycoAlert Assay Control Set Lonza, Walkersville MD Cat # LT07-518 NEBuilder HiFi DNA Assembly Master Mix New England Biolabs Cat# E2621S Blood and Cell Culture DNA Mini kit Qiagen Cat# 13323 Deposited data WGS Data This paper https://www.ncbi.nlm.nih.gov/sra/SRA BioProject ID PRJNA1074863 Cryo EM structure of Pf20S proteasome binding to SW584 This paper https://www.rcsb.org PDB ID 8W2F; https://www.ebi.ac. uk/emdb/EMDB (EMD-43746) Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome (Pf20SPP1, 20–30 kdal band; pooled Ni+2 column fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD052762 Mass spectrometry LC-MS/MS proteomics data on the purified Pf20S proteasome Ni+2 column (Pf20SPP1, 10–15 kdal band; pooled Ni+2 column fraction; LUM1_1032594) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Mass spectrometry SRM proteomics data on the purified Pf20S proteasome used for kinetic analysis (Pf20SPP1; pooled gel filtration fraction) This paper PRIDE partner repository http://www.ebi.ac.uk/pride PXD052830 Mass spectrometry LC-MS/MS proteomics data of the purified Pf20S proteasome used for structural and kinetic analysis (Pf20SPP2; pooled gel filtration fraction; LUM1_1060117) This paper PRIDE partner repository http://www.ebi.ac.uk/pride ProteomeXchange; PXD053252 Experimental models: Cell lines P. falciparum line Dd2 BEI resources Cat# Dd2-MRA-102 P. falciparum line 3D7 BEI resources Cat# 3D7-MRA-845 P. falciparum line NF54HT-GFP-luc BEI resources Cat # NF54-MRA-1217 P. falciparum line 3D7 (His-Tagged 20S- b7) CRISPR This paper N/A (Continued on next page) Cell Chemical Biology 31, 1–15.e1–e19, August 15, 2024 e2 ..



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